superscript iii pre-amplification kit Search Results


ultra dna library prep kit  (New England Biolabs)
98
New England Biolabs ultra dna library prep kit
Ultra Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
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mir x mirna qrt pcr sybr kit  (TaKaRa)
96
TaKaRa mir x mirna qrt pcr sybr kit
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Mir X Mirna Qrt Pcr Sybr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nebnext ultra ii dna library prep kit  (New England Biolabs)
99
New England Biolabs nebnext ultra ii dna library prep kit
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Nebnext Ultra Ii Dna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra ii dna library prep kit/product/New England Biolabs
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ctc grandperformance assays  (TATAA Biocenter AB)
90
TATAA Biocenter AB ctc grandperformance assays
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Ctc Grandperformance Assays, supplied by TATAA Biocenter AB, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
ctc grandperformance assays - by Bioz Stars, 2026-05
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taqman gene expression assay kit  (Thermo Fisher)
96
Thermo Fisher taqman gene expression assay kit
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Taqman Gene Expression Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
taqman gene expression assay kit - by Bioz Stars, 2026-05
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genomiphi hy dna amplification kit  (GE Healthcare)
93
GE Healthcare genomiphi hy dna amplification kit
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Genomiphi Hy Dna Amplification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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taqman preamp master mix kit  (Thermo Fisher)
96
Thermo Fisher taqman preamp master mix kit
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Taqman Preamp Master Mix Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/taqman preamp master mix kit/product/Thermo Fisher
Average 96 stars, based on 1 article reviews
taqman preamp master mix kit - by Bioz Stars, 2026-05
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alkaline phosphatase  (New England Biolabs)
98
New England Biolabs alkaline phosphatase
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Alkaline Phosphatase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alkaline phosphatase/product/New England Biolabs
Average 98 stars, based on 1 article reviews
alkaline phosphatase - by Bioz Stars, 2026-05
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nebnext kits  (New England Biolabs)
96
New England Biolabs nebnext kits
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Nebnext Kits, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vahts universal dna library prep kit  (Vazyme Biotech Co)
96
Vazyme Biotech Co vahts universal dna library prep kit
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Vahts Universal Dna Library Prep Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vahts universal dna library prep kit/product/Vazyme Biotech Co
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superscript ii dna preamplification kit  (Thermo Fisher)
99
Thermo Fisher superscript ii dna preamplification kit
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Superscript Ii Dna Preamplification Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/superscript ii dna preamplification kit/product/Thermo Fisher
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nebnext ultra ii rna library prep kit  (New England Biolabs)
99
New England Biolabs nebnext ultra ii rna library prep kit
( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with <t>qRT-PCR.</t> The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).
Nebnext Ultra Ii Rna Library Prep Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nebnext ultra ii rna library prep kit/product/New England Biolabs
Average 99 stars, based on 1 article reviews
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Image Search Results


( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with qRT-PCR. The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).

Journal: Scientific Reports

Article Title: Optimization of CRISPR/Cas9 genome editing to modify abiotic stress responses in plants

doi: 10.1038/srep26685

Figure Lengend Snippet: ( a ) Various types of CRISPR/Cas9-induced mutation detected by amplicon sequencing in T1 plants. The red boxes indicate target sites and green characters are PAM sequences. The blue characters show the nucleotide insertion. L1-7; the individual T1 lines for the transgenic plants. ( b ) The mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. ( c ) The detection of sgRNA expression levels in individual T1 Arabidopsis plants with each sgRNA specific primer with qRT-PCR. The value for one of the T1 lines of each construct was set to 1.0. To normalize the expression levels, 18S rRNA was amplified as an internal control. WT; wild-type Arabidopsis. ( d ) Protein blot analysis of GFP and Cas9 was performed with the individual T1 plants using an anti-GFP and Cas9 antibody, respectively. ( e ) The off-target mutation rates analyzed by next-generation amplicon-based deep sequencing using mixed DNA pools from 15–30 GFP-positive T1 plants. The off-target rates were shown by calculating the differences between these values and those from wild-type Arabidopsis (data not shown).

Article Snippet: Mir-X™ miRNA qRT-PCR SYBR Kit (Takara, Japan) was used for the isolation of sgRNA expressed in plant cells.

Techniques: CRISPR, Mutagenesis, Amplification, Sequencing, Transgenic Assay, Expressing, Quantitative RT-PCR, Construct